Journal: Scientific Reports

Article Title: Microfluidic control of axonal guidance

doi: 10.1038/srep06457

Figure Lengend Snippet: (a) Petridish-view of microfluidic guidance setup. Green cylinder indicates initial orientation of axonal and growth cone, while green arrow indicates initial outgrowth direction. Red cylinder indicates final orientation of axonal shaft and growth subsequent to application of microfluidic flow (Blue arrow: flow rate: 2.5 μL/min). (b) Histogram of axonal turning angle in absence (control) and presence of microfluidic flow at two different time points (n = 8). The error bars around mean represent standard error of the mean. (c) Simulation of radial force distribution on axon induced by microfluidic flow at various axial positions, (d) estimated total force on axonal elements induced by microfluidic flow along length of axon. Magnitude of force is depicted by length of arrow.

Article Snippet: To determine the total force exerted by the flow on the axon, finite-element simulation of the microfluidic flow was carried out in ANSYS-CFX.

Techniques: Control

Journal: Scientific Reports

Article Title: Microfluidic control of axonal guidance

doi: 10.1038/srep06457

Figure Lengend Snippet: (a–f) Time-lapse images showing significant deviation of the direction of growth cone migration in response to microfluidic flow. The direction of flow is marked by white arrow. Bar: 20 μm. (g–i) Sequence of overlay profiles depicting the directional change of axonal growth in pseudocolor from 0 to 70 min.

Article Snippet: To determine the total force exerted by the flow on the axon, finite-element simulation of the microfluidic flow was carried out in ANSYS-CFX.

Techniques: Migration, Sequencing

Journal: Scientific Reports

Article Title: Microfluidic control of axonal guidance

doi: 10.1038/srep06457

Figure Lengend Snippet: (a) Average kinetics of advancing axon's turning angle in response to microfluidic flow (n = 8). (b) Axonal growth kinetics during microfluidic guidance (n = 8). The error bars around mean represent standard error of the mean. (c) Cumulative distribution of turning (angle) of the axon during microfluidic flow (n = 8). (d) Theoretically predicted final position of axon after bending under application of a distributed force for an axon having 10, 40, 70 and 100 microtubules.

Article Snippet: To determine the total force exerted by the flow on the axon, finite-element simulation of the microfluidic flow was carried out in ANSYS-CFX.

Techniques:

Journal: Scientific Reports

Article Title: Microfluidic control of axonal guidance

doi: 10.1038/srep06457

Figure Lengend Snippet: (a–e) Time-lapse images of turning of growth cone in response to direct microfluidic flow. The direction of flow is marked by red arrow, the angle between original growth direction and flow direction being ~90°. (f–h) Fasciculation of guided axon over another axon. Scale bar: 50 μm. (i) Overlapped outline of microfluidic flow assisted axonal turning and fasciculation process. Vertical axis represents initial outgrowth direction. Blue arrows illustrate fluid flow profile. (j) Kinetics of turning (angle) of the growth cone during microfluidic flow. (k) Growth rate of axon at different time points during turning.

Article Snippet: To determine the total force exerted by the flow on the axon, finite-element simulation of the microfluidic flow was carried out in ANSYS-CFX.

Techniques: